Afp4® Screening - Quadruple Marker Test (AFP, hCG, uE3, Inhibin A)
82105; 82677; 84702; 86336
AFP Tetra; Alpha-Fetoprotein (AFP) Tetra Profile;
α-Fetoprotein, serum; dimeric inhibin A (DIA); human chorionic gonadotropin (hCG); unconjugated estriol (uE3)
Please Note: Ultrasound BPD (biparietal diameter) dating of pregnancy is preferred for optimal accuracy of risk calculations.
Expected Turnaround Time
2 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Whole blood; serum
Serum: 2-3 mL (unhemolyzed).
Whole Blood: 7 mL in SST or 10 ml in red-top tube; allow to clot; centrifuge (15 minutes at 1,000 g) to prevent hemolysis.
Serum: AFP tube
Whole Blood: Serum separator tube (SST) or red-top tube.
Avoid hemolysis. Send complete specimen in the original tube. Do not pour off.
Maintain at room temperature
Causes for Rejection
Gross hemolysis; gross lipemia; quantity not sufficient for analysis
Screening test for open neural tube defects (detects 80% open spina bifida, 90% anencephaly), Down syndrome (detects 75% to 80%), and trisomy 18 (detects 73%)
Immunochemiluminometric assay (ICMA)